Experimental study of combined effect of Nd:YAG laser endodissector and biostimulating low-energy He-Ne laser on retinal pigment epithelium cell culture

Abstract

Development and adaptation of more effective, safety and available methods of eye microsurgery is an important objective in modern ophthalmology. The conducted in vitro studyshowed that combined use ofhigh-energy Nd:YAG endodissector (1,44 mkm) and biostimulating low-energy beam of He-Ne laser (632 nm) delivered with one light guide, has a posllive effect on monolayer cell cullure of retinal pigment epllhelium. The absence of changes in cell phenotype and high proliferative activity demonstrates stimulating effect of the studied beam. It was shown that in experimental groups independent of irradiation time synthesis of anti-inflammatory proteins cyclooxygenase-2 and prostaglandine-2 was induced, which resulted in activation of reparative processes, downregulation of pathological changes both on cellular and tissue-organ level.