Comparative study of p199 effect on 2D and 3D cultures of human dermal fibroblasts

Abstract

Nowadays, biotechnologies open the way for application of a wide range of effective and innovative cosmetic active substances. Modern development of cell technologies theoretically allows us to completely replace animal models for the development and analysis of cosmetic products with cell cultures, which are relatively easy-to-use, highly sensitive to toxic effects, and preferable in the context of bioethics. Monolayer culture of dermal fibroblasts is a widely used model system in the field of testing and development of cosmetic products. In contrast to 2D monolayer, 3D spheroids from dermal fibroblasts can become a model that would be closer to native tissue organization while maintaining a high throughput analysis capacity. In the current study, we compared the dose-dependent effect of P199 peptide on the expression of I, III and IV types collagen and fibronectin in 2D and 3D cultures of human dermal fibroblasts. By using this three-dimensional model, we were able to evaluate changes in the level of expression of extracellular matrix proteins, as well as their structure during synthesis to the extracellular space.