Molecular correlates of acute myocardial infarction in experiments and clinical setting

Abstract

Objective: to model ischemic myocardial infarction in rats, to assess its severity using in stru mental methods, and to compare the detected shifts in serum homeostasis with the changes observed in patients with infarction in clinical settings. Methods: modeling of myocardial infarction by irreversible occlusion of the descending branch of the left coronary artery, enzyme immunoassay, histology, morphometry, and laser correlation spectroscopy. Results: the adequacy of the simulation in rats was confirmed by increased serum content of brain natriuretic peptide and characteristic changes in the myocardium detected by histological and morphometric methods. The serum subfractional composition of experimental animals and patients with myocardial infarction was demonstrated to be different from the control. Conclusion: laser correlation spectroscopy may be a promising method to supplement generally accepted diagnostic methods and can be used to assess the effectiveness of administered therapy.